Composite

Part:BBa_K5425005:Design

Designed by: Sampoorn Pandey   Group: iGEM24_IISER-Kolkata   (2024-09-21)


This circuit was designed to determine the threshold population at which the pLux promoter becomes a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2493
    Illegal AgeI site found at 3539
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While designing this part we specifically used sfGFP due to is faster folding time for characterisation. But we didn't add degradation tag to the flourophore which we felt was essential after conducting the experiments.


Source

All the sequences were sourced either from NCBI or the Parts Registry and then assembled in silico using SnapGene.

References